SFB796 - Central project Z1 :  Analytics & Imaging

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Extended Summary

The overall aim of the Z1 project is to provide an integrated platform of complementary techniques to support the research pursued within the individual sub-projects of the CRC796. The Z1 project is divided into five different modules:

Module I -   Bioinformatics
Module I provides a central computational platform for the analysis of microbial effectors and their cellular targets. The work packages are embedded into subproject A2 in which computational methods have been optimized that allow the prediction of linear sequence motifs in microbial effectors that could mediate protein-protein interactions and thus are involved in effector function. As in the first funding period, costs for personnel are defrayed by project A2 which also lists the detailed work plan (see project description A2, H. Sticht).

Module II -   Protein production and protein crystallography
The central goal of this module is to provide a platform for the heterologous production of proteins of interest, to validate the native-folded state of these proteins and to determine individual conditions for the successful crystallisation and subsequent crystal structure determination. In contrast to the previous funding period the production of proteins in E. coli will predominantly be achieved in the individual projects, whereas module II will focus on the heterologous production of proteins in eukaryotic cells, the labelling of proteins for solving the crystallographic phase problem or NMR structure determination, the biophysical characterisation and the crystallisation of protein samples.

Module III -   Bioimaging
Module III continues to provide state-of-the-art imaging technologies, including confocal microscopy and life-cell-imaging. Becoming effective as of the second half of 2012 the platform will be transferred into the newly established Optical Imaging Centre Erlangen (OICE) which bundles the available expertise in bio-imaging creating an added value for the CRC as researchers will gain access to novel imaging methods and technology platforms previously not available to the consortium. Access to the platform will be organized using the online microscope booking system established during the first funding period. Members of the CRC will also receive scientific support and user training through the OICE.

Module IV -   Yeast two-hybrid screenings
Yeast two-hybrid screenings and initial down-stream analysis of positive clones will be provided as a service. As in the previous funding period necessary resources will be provided.

Module V -   Proteomics platform
Aim of Module V is to provide comprehensive and efficient service in MS-based proteomics analyses. The module will offer mass-spectrometric analyses of proteins with a particular focus on protein identification in purified protein complexes and the characterization of post-translational modifications. To achieve this goal state-of-the-art instrumentation and guidance by experts in MS-based proteomics analyses will be made available to the scientists within the CRC796.